Journal: Oncotarget

Article Title: New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams

doi: 10.18632/oncotarget.12456

Figure Lengend Snippet: ( A ) Representative image of BM-MSCs 5 days after seeding. ( B – D ) Characterization of BM-MSCs by flow cytometry. The majority of the cells are CD105 + , CD44 + and CD29 + , which are typical characteristic phenotypes of BM-MSCs. ( E ) BM-MSC can be differentiated into adipogenic and myogenic lineages. Adipogenic differentiation was characterized by Oil Red O staining, while myogenic differentiation was evidenced by the formation of myotubes stained with crystal violet. ( F ) Representative image of immunostaining of BM-MSCs on GF scaffold, stained by anti-β-tubulin (green) and DAPI for nucleus (blue). Effects of different concentrations of VC (5 to 100 μg/ml, 5 days) ( G ) and H 2 O 2 exposure (0.1 to 2 mM, 24 hours) ( H ) on cell viability of BM-MSCs cultured for 5 days, measured by MTT assay. ( I ) Cell viability of BM-MSCs in the five experimental groups. Data were presented as mean ± SEM. * p < 0.05 vs control.

Article Snippet: Antibodies used in the study were as follows: CD105-FITC (MCA1557FT, Serotec, Oxford, UK), CD44-FITC (MCA643FA, Serotec), and CD29-FITC (MCA1949FT, Serotec).

Techniques: Flow Cytometry, Staining, Immunostaining, Cell Culture, MTT Assay, Control

Journal: Cytotechnology

Article Title: Role of nanoparticles in osteogenic differentiation of bone marrow mesenchymal stem cells

doi: 10.1007/s10616-019-00353-y

Figure Lengend Snippet: Flow cytometry analysis of BM-MSCs a cluster of differentiation (CD) 73 (89.8%), b CD105 (85.7%), c CD90 (83.5%), d CD34 (8.1%) and e CD45 (0.6%), antibodies; where CD73, CD105 and CD90 stand for the fluorescent-tagged antibodies used to detect the surface antigens that are positively expressed on mesenchymal stem cells (MSCs), while CD34 and CD45 stand for the fluorescent-tagged antibodies used to detect the cell surface antigens that are only expressed on the hematopoietic stem cells and are negatively expressed on MSCs. The horizontal line in each panel represents the channel in the flow cytometry that detects the fluorescent-tagged antibodies used for detection of the expression of these markers on the MSCs

Article Snippet: In brief, BM-MSCs of third passage were released from culture flasks using trypsin/EDTA digestion, suspended in PBS containing 0.5% bovine serum albumin (BSA)/2 mmol EDTA and analyzed for surface markers expression using anti-rat phycoerythrin (PE)-conjugated CD73 and anti-rat PE-conjugated CD90 antibody (R&D Systems,UK), anti-rat fluorescein isothiocyanate (FITC)-labeled CD105 antibody (MiltenyiBiotec, Germany), anti-rat FITC-labeled CD45 antibody and anti-rat PE-conjugated CD34 antibody (Beckman Coulter Co.,USA).

Techniques: Flow Cytometry, Expressing

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD105 (REA1058) FITC , Miltenyi Biotec , 130-118-173; RRID: AB_2733613.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: Laryngoscope Investigative Otolaryngology

Article Title: On the in vivo origin of human nasal mesenchymal stem cell cultures

doi: 10.1002/lio2.472

Figure Lengend Snippet: Antibodies used for immunofluorescence

Article Snippet: Mouse anti‐CD105 , Bio‐Rad , Cat# MCA1557T; AB_1100559 , 1:50.

Techniques:

Journal: Applied sciences (Basel, Switzerland)

Article Title: Hypoxia Modulates Regenerative Potential of Fetal Stem Cells

doi: 10.3390/app12010363

Figure Lengend Snippet: Evaluation of stemness gene expression, proliferation potential, and surface marker expression in fetal cells following either normoxia (N) or hypoxia (L) incubation. ( A ) Fetal NPC and SDSC cell morphology during expansion. ( B ) RT-qPCR assessed the expression of stemness genes ( MYC , KLF4 , BMI1 , POU5F1 , NES , NOV , NANOG , and SOX2 ) in both fetal cell types after hypoxia treatment, normalized against GAPDH levels as an internal control. Data ( n = 4) are represented in bar charts. Different letters indicate a statistically significant difference compared to the groups within the same gene type ( p < 0.05). Flow cytometry assessed the positive percentage and median intensity of relative EdU incorporation ( C ) and expression of MSC surface markers SSEA4 ( D ), CD73 ( E ), CD90 ( F ), CD105 ( G ), and CD146 ( H ).

Article Snippet: Cell surface marker expression was evaluated using the following primary antibodies: the stage-specific embryonic antigen 4-PE (SSEA4-PE; BioLegend, Dedham, MA, USA), CD73-APC (Thermo Fisher Scientific), CD90-APC-Vio770 (Miltenyi Biotec, San Diego, CA, USA), CD105-PerCP-Vio700 (Miltenyi Biotec), and CD146-PE (Thermo Fisher Scientific).

Techniques: Gene Expression, Marker, Expressing, Incubation, Quantitative RT-PCR, Control, Flow Cytometry